How To Obtain Spore Prints

In many cases it is desirable to obtain
spores in a mass on paper in order to know the exact tint of color
produced by the species.

Often the color of the spores can be
satisfactorily determined by an examination of them under the
microscope. One cannot always depend on the color of the lamellæ since a
number of the species possess colored cystidia or spines in the hymenium
which disguise the color of the spores.

The best way to determine the color of the spores in mass is to catch them as they fall from the
fruiting surface on paper. For the ordinary purpose of study and
reference in the herbarium the spores caught on unprepared paper, which
later may be placed in the packet with the specimen, will answer. This
method has the advantage of saving time, and also the danger of injury
to the spores from some of the fixatives on prepared paper is avoided.

If for purposes of illustration one wishes pretty spore prints, perfect
caps must be cut from the stem and placed fruiting surface downward on
paper prepared with some gum arable or similar preparation spread over
it, while the paper is still moist with the fixative, and then the
specimen must be covered with a bell-jar or other receiver to prevent
even the slightest draft of air, otherwise the spores will float around more or less.

The spores may be caught on a thin, absorbent paper, and
the paper then be floated on the fixative in a shallow vessel until it
soaks through and comes in contact with the spores. I have sometimes
used white of egg as a fixative. These pieces of paper can then be cut
out and either glued to card-boards, or onto the herbarium sheet.



Powered by WordPress Lab